Comparative Study of Amyloidogenic Potential of Agno3 and Freund's Adjuvant (af) with That of Vitamin Free Casein, on Spatio-temporal Pattern of Experimental Amyloidosis in Mice

ì õ } . Abstract Reactive amyloidosis is a condition that complicates a long list of chronic inflammation, chronic infectious, malignant, and hereditary disorders. In the present study the potential effects of two amyloidogenic substances: ie. AgNO3 and Freund's Adjuvant (AF) with that of vitamin free casein, on spatio-temporal pattern of experimental amyloidosis in mice were compared. For this purpose a total of 40 male Swees mice, obtained from Pasteur Institute Tehran, after being weighted were randomly divided into 4 groups including 2 treatments, 1 control (vitamin free casein) and 1 positive control (normal saline). At the end of 3rd, 5th and 7th weeks of experiment 3 mice were randomly selected and euthnised. Spleen sample of each animal obtained and preserved in 10% neutral buffer formalin. Sample were then processed through different stages of dehydration, clearing and impregnation and finally embedded in paraffin blocks. Sections of 5 m thickness then cut and stained by alkaline Congo red techniques. Spleen weights and the data obtained from microscopic quantitative analysis did show no significant differences between groups A and B, A and C, and B and C. But significant differences were observed between groups A and D, B and D, and C and D respectively. It is concluded that two compounds ie; AgNO3 and Freund's Adjuvant have the same potential, as does vitamin free casein have, in spatio temporal pattern of experimental amyloidosis in mice. Islamic.Azad.Univ., Garmsar Branch. 5,1:67-71,2009 .